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Thermal denaturation of rSBA and gSBA Thermal unfolding profiles of both nonglycosylated and glycosylated SBA were monitored by the change in molar ellipticity value at 218 nm at a protein concentration of 13 µM
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A further note of importance is that for the CPMG settings used, R^sub 2^(δ^sub 1^) is measured in the high ω^sub 1eff^ limit (ω^sub 1eff^ > p^sub A^Δω^sub I^), so that R^sub ex^ is proportional to B^sup 2^^sub o^ independent of whether the exchange is fast or slow to a skewed population (33,34)
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Mass spectrometryThe BLG preparation was examined by liquid chromatography-mass spectroscopy using reverse phase chromatography with on-line electrospray-ionization mass spectrometry (MicroMass, Cary, NC) as described previously (14)
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8 µM for ThT (Fig
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For typical membrane concentrations used in these studies, the apparent K(d) should be ~200-fold stronger than the true K(d) (see Runnels and Scarlata, 1999)
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2 B); incubation for longer time (2
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